overlay_bigwig.py¶

Perform pairwise operations on two BigWig files. Both files must use the same reference genome.

Usage¶

overlay_bigwig.py -i file1.bw -j file2.bw -a mean -o output.wig

Option	Description	Default
`-i`, `--bwfile1`	First BigWig file	Required
`-j`, `--bwfile2`	Second BigWig file	Required
`-a`, `--action`	Operation: `mean`, `sum`, `diff`, `ratio`, `max`, `min`, `geometric_mean`	Required
`-o`, `--output`	Output wiggle file	Required
`-c`, `--chunk`	Chromosome chunk size (bp) for processing	100000

Wiggle file with the result of the pairwise operation.